Last Wednesday I had a meeting with the folks of the New Zealand Drylands Forest Initiative in Blenheim. In addition to sitting in a conference room and having nice sandwiches we went to visit one of our progeny trials at Cravens. Plantation forestry trials are usually laid out following a rectangular lattice defined by rows and columns. The trial follows an incomplete block design with 148 blocks and is testing 60 *Eucalyptus bosistoana* families. A quick look at survival shows an interesting trend: the bottom of the trial was much more affected by frost than the top.

setwd('~/Dropbox/quantumforest') library(ggplot2) # for qplot library(asreml) load('cravens.Rdata') qplot(col, row, fill = Surv, geom='tile', data = cravens)

We have the trial assessed for tree height (ht) at 2 years of age, where a plot also shows some spatial trends, with better growth on the top-left corner; this is the trait that I will analyze here.

Tree height can be expressed as a function of an overall constant, random block effects, random family effects and a normally distributed residual (this is our base model, m1). I will then take into account the position of the trees (expressed as rows and columns within the trial) to fit spatially correlated residuals (m2)—using separable rows and columns processes—to finally add a spatially independent residual to the mix (m3).

# Base model (non-spatial) m1 <- asreml(ht ~ 1, random = ~ Block + Family, data = cravens) summary(m1)$loglik [1] -8834.071 summary(m1)$varcomp gamma component std.error z.ratio constraint Block!Block.var 0.52606739 1227.4058 168.84681 7.269345 Positive Family!Family.var 0.06257139 145.9898 42.20675 3.458921 Positive R!variance 1.00000000 2333.1722 78.32733 29.787459 Positive

`m1`

represents a traditional family model with only the overall constant as the only fixed effect and a diagonal residual matrix (identity times the residual variance). In `m2`

I am modeling the R matrix as the Kronecker product of two separable autoregressive processes (one in rows and one in columns) times a spatial residual.

# Spatial model (without non-spatial residual) m2 <- asreml(ht ~ 1, random = ~ Block + Family, rcov = ~ ar1(row):ar1(col), data = cravens[order(cravens$row, cravens$col),]) summary(m2)$loglik [1] -8782.112 summary(m2)$varcomp gamma component std.error z.ratio Block!Block.var 0.42232303 1025.9588216 157.00799442 6.534437 Family!Family.var 0.05848639 142.0822874 40.20346956 3.534080 R!variance 1.00000000 2429.3224308 88.83064209 27.347798 R!row.cor 0.09915320 0.0991532 0.02981808 3.325271 R!col.cor 0.28044024 0.2804402 0.02605972 10.761445 constraint Block!Block.var Positive Family!Family.var Positive R!variance Positive R!row.cor Unconstrained R!col.cor Unconstrained

Adding two parameters to the model results in improving the log-likelihood from -8834.071 to -8782.112, a difference of 51.959, but with what appears to be a low correlation in both directions. In `m3`

I am adding an spatially independent residual (using the keyword `units`

), improving log-likelihood from -8782.112 to -8660.411: not too shabby.

m3 <- asreml(ht ~ 1, random = ~ Block + Family + units, rcov = ~ ar1(row):ar1(col), data = cravens[order(cravens$row, cravens$col),]) summary(m3)$loglik [1] -8660.411 summary(m3)$varcomp gamma component std.error z.ratio Block!Block.var 3.069864e-07 4.155431e-04 6.676718e-05 6.223763 Family!Family.var 1.205382e-01 1.631630e+02 4.327885e+01 3.770040 units!units.var 1.354166e+00 1.833027e+03 7.085134e+01 25.871456 R!variance 1.000000e+00 1.353621e+03 2.174923e+02 6.223763 R!row.cor 7.814065e-01 7.814065e-01 4.355976e-02 17.938724 R!col.cor 9.529984e-01 9.529984e-01 9.100529e-03 104.719017 constraint Block!Block.var Boundary Family!Family.var Positive units!units.var Positive R!variance Positive R!row.cor Unconstrained R!col.cor Unconstrained

Allowing for the independent residual (in addition to the spatial one) has permitted higher autocorrelations (particularly in columns), while the Block variance has gone very close to zero. Most of the Block variation is being captured now through the residual matrix (in the rows and columns autoregressive correlations), but we are going to keep it in the model, as it represents a restriction to the randomization process. In addition to log-likelihood (or AIC) we can get graphical descriptions of the fit by plotting the empirical semi-variogram as in `plot(variogram(m3))`

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## 0 responses to “Spatial correlation in designed experiments”

Great work! Would you mind showing the plots of adjusted values (BLUPs)? It would be very nice to see how the patterns got adjusted by including the row and column effects. Thank you very much.

Hi Luis, this is an interesting example. I’m also using ASreml-R (v4) to try and analyze spatial data in a loblolly pine trial in east Texas. My trial has gaps in it where blocks have not been sampled, and some missing trees within the sampled reps (I wasn’t responsible for this). How does your Cravens analysis deal with NA values? There is no argument for and na.action. Thank you!